Journal: Nanomaterials

Article Title: ZnO Nanoparticles Induce Dyslipidemia and Atherosclerotic Lesions Leading to Changes in Vascular Contractility and Cannabinoid Receptors Expression as Well as Increased Blood Pressure

doi: 10.3390/nano11092319

Figure Lengend Snippet: Effect of ZnONPs on aorta contractility. ( a ) Effect of ACPA (CB 1 receptor agonist) on contraction in aortic rings in different experimental groups. ( b ) Effect of HU308 (CB 2 receptor agonist) on contraction in aortic rings in different experimental groups. Blue bars correspond to the control non-treated group, green bars correspond to the ZnONPs treated-groups at different times; ( a ) there is a significant difference compared to the phenylephrine group with p < 0.05; ( b ) there is a significant difference compared to the control group with p < 0.05.

Article Snippet: Next, aorta sections were blocked (Animal-free blocker and diluent, Vector laboratories Inc., Burlingame, CA, USA) and incubated with rabbit polyclonal Anti-CB 1 (1:100, Alomone, Labs, Jerusalem, Israel) or rabbit polyclonal Anti-CB 2 (1:100, Alomone, Labs, Jerusalem, Israel) primary antibody and co-labeled with mouse monoclonal Anti-alpha smooth muscle Actin antibody (1:200, Abcam, Cambrige, UK) overnight.

Techniques:

Journal: Nanomaterials

Article Title: ZnO Nanoparticles Induce Dyslipidemia and Atherosclerotic Lesions Leading to Changes in Vascular Contractility and Cannabinoid Receptors Expression as Well as Increased Blood Pressure

doi: 10.3390/nano11092319

Figure Lengend Snippet: Effect of ZnONPs on the CB 1 and CB 2 receptors expression in the aorta wall. ( a ) CB 1 expression, ( b ) CB 2 expression. Blue bars correspond to the control non-treated group, green bars correspond to the ZnONPs treated-groups at different times; ( a ) there is a significant difference compared to the control group with p < 0.05.

Article Snippet: Next, aorta sections were blocked (Animal-free blocker and diluent, Vector laboratories Inc., Burlingame, CA, USA) and incubated with rabbit polyclonal Anti-CB 1 (1:100, Alomone, Labs, Jerusalem, Israel) or rabbit polyclonal Anti-CB 2 (1:100, Alomone, Labs, Jerusalem, Israel) primary antibody and co-labeled with mouse monoclonal Anti-alpha smooth muscle Actin antibody (1:200, Abcam, Cambrige, UK) overnight.

Techniques: Expressing

Journal: Nanomaterials

Article Title: ZnO Nanoparticles Induce Dyslipidemia and Atherosclerotic Lesions Leading to Changes in Vascular Contractility and Cannabinoid Receptors Expression as Well as Increased Blood Pressure

doi: 10.3390/nano11092319

Figure Lengend Snippet: Representative images showed ZnONPs effect on CB 1 and CB 2 receptors expression in aorta wall. Transmitted light images (grey). Scale bar corresponds to 50 μm. Smooth muscle α-actin was detected with a specific antibody labeled with Alexa Fluor 568 (red). CB 1 and CB 2 located on the aorta ring were detected by specific antibodies and labeled with FITC (green). Image overlap indicates a high degree of colocalization of CB 1 or CB 2, and smooth muscle α-actin (yellow). Nuclei were counterstained with DAPI dye (blue).

Article Snippet: Next, aorta sections were blocked (Animal-free blocker and diluent, Vector laboratories Inc., Burlingame, CA, USA) and incubated with rabbit polyclonal Anti-CB 1 (1:100, Alomone, Labs, Jerusalem, Israel) or rabbit polyclonal Anti-CB 2 (1:100, Alomone, Labs, Jerusalem, Israel) primary antibody and co-labeled with mouse monoclonal Anti-alpha smooth muscle Actin antibody (1:200, Abcam, Cambrige, UK) overnight.

Techniques: Expressing, Labeling

Journal: International Journal of Molecular Sciences

Article Title: Effects of Rare Phytocannabinoids on the Endocannabinoid System of Human Keratinocytes

doi: 10.3390/ijms23105430

Figure Lengend Snippet: List of antibodies used for Western blot analysis.

Article Snippet: CB 2 receptor rabbit polyclonal , 1:200 , Cayman Chemical (Ann Arbor, MI, USA).

Techniques: Western Blot

Journal: Brazilian Journal of Medical and Biological Research

Article Title: Kahweol, a natural diterpene from coffee, induces peripheral antinociception by endocannabinoid system activation

doi: 10.1590/1414-431X2021e11071

Figure Lengend Snippet: Schematic diagram of the experimental protocol. A , Kahweol (Kah) was administered 175 min after the local administration of prostaglandin E 2 (PGE 2 ) (2 µg) and the antinociceptive response was measured prior to and 180 min, 195 min, and 210 min after PGE 2 injection. B , Kah was administered in the right hind paw 175 min after local injection of PGE 2 . The cannabinoid drugs AM251, AM630, MAFP, JZL184, or VDM11 were given 10 min prior (165 min) to Kah intraplantar administration and measurements were performed prior to and 180 min after PGE 2 administration.

Article Snippet: The CB 1 cannabinoid receptor antagonist AM251 ( N -[piperidin-1-yl]-5-[4-iodophenyl]-1-[2,4-dichlorophenyl]-4-methyl-1 H -pyrazole-3-carboxamide; purity>99%; Tocris) (20, 40, 80 μg/paw) and the CB 2 cannabinoid receptor antagonist AM630 (6-Iodo-2-methyl-1-[2-{4-morpholinyl}ethyl]-1H-indol-3-yl [4-ethoxyphenyl] methanone; purity >98%; Tocris) (100 μg/paw) were dissolved in 10% DMSO, whereas the hyperalgesic agent PGE 2 (purity ≥93%; Sigma-Aldrich, USA) was dissolved in 2% ethanol.

Techniques: Injection

Journal: Brazilian Journal of Medical and Biological Research

Article Title: Kahweol, a natural diterpene from coffee, induces peripheral antinociception by endocannabinoid system activation

doi: 10.1590/1414-431X2021e11071

Figure Lengend Snippet: The CB 2 receptor antagonist did not block kahweol-induced peripheral antinociception in hyperalgesic paws. The antinociceptive response was measured by the paw pressure test. Prostaglandin E 2 (PGE 2 ) injection (2 µg/paw) was done at time 0, AM630 (100 µg/paw) was injected at time 165 min, and kahweol (Kah; 80 µg/paw) was given at 175 min. Measurements were made prior to and 180 min after PGE 2 administration. Data are reported as means±SE (n=5) of Δ nociceptive threshold measured in grams (g). *P<0.05 compared to PGE 2 + Veh 2 + Veh 3-injected group (ANOVA and Bonferroni's test). There was no significant difference between (PGE 2 + Veh 2 + Kah 80) and (PGE 2 + AM630 100 + Kah 80)-injected groups. Veh (vehicle) 2: 10% DMSO in saline; Veh 3: sterile saline solution (0.9% NaCl).

Article Snippet: The CB 1 cannabinoid receptor antagonist AM251 ( N -[piperidin-1-yl]-5-[4-iodophenyl]-1-[2,4-dichlorophenyl]-4-methyl-1 H -pyrazole-3-carboxamide; purity>99%; Tocris) (20, 40, 80 μg/paw) and the CB 2 cannabinoid receptor antagonist AM630 (6-Iodo-2-methyl-1-[2-{4-morpholinyl}ethyl]-1H-indol-3-yl [4-ethoxyphenyl] methanone; purity >98%; Tocris) (100 μg/paw) were dissolved in 10% DMSO, whereas the hyperalgesic agent PGE 2 (purity ≥93%; Sigma-Aldrich, USA) was dissolved in 2% ethanol.

Techniques: Blocking Assay, Randall–Selitto Test, Injection, Saline, Sterility